In both cell lines, induction of cell death was induced in sh-scrambled controls, whereas the knockdown of endogenously induced FOXO1 significantly reduced the apoptotic MSA effect, suggesting that FOXO1 is at least partially involved in MSA-induced cell death (Figure 6d).To assess the potential of MSA to reduce ES tumor growth in vivo, we used an orthotopic mouse xenotransplantation model. The gene discussed is FOXO1; the disease is Ewing sarcoma.