In both cell lines, induction of cell death was induced in sh-scrambled controls, whereas the knockdown of endogenously induced FOXO1 significantly reduced the apoptotic MSA effect, suggesting that FOXO1 is at least partially involved in MSA-induced cell death (Figure 6d).To assess the potential of MSA to reduce ES tumor growth in vivo, we used an orthotopic mouse xenotransplantation model. This evidence concerns the gene FOXO1 and neoplasm.