In this work, we initially analysed the expression of PSA and PSMA according to RKIP and then investigated the components of Ras/Raf-1/MEK/ERK, NF-κB (p50/p65), and Akt (T308/S473) pathways in conjunction with PSA and PSMA according to RKIP in human cancerous prostate with (PSA+, PSMA+) profile to assess the biological characters of this mostly immunoexpressed PC profile. This evidence concerns the gene NFKB1 and pachyonychia congenita.