This study was performed in prostate cancer tissues which were evaluated for PSA, PSMA, RKIP, Raf-1, MEK-1, ERK1/2, p-Akt (T308/S473), and NF-κB (p50/p65) expression by immunohistochemistry analysis using isoform-specific antibodies. This evidence concerns the gene MAP2K1 and Familial prostate cancer.