Hierarchical clustering summarized our biochemical results and confirmed three HCC cell line groups (Figure 7): (1) HepG2, Hep3B, HuH7, PLC; (2) HLE, HLF, FLC-4, HuH6 as well as (3) HCC-M plus HCC-T. The gene discussed is HSPG2; the disease is hepatocellular carcinoma.