High-throughput strand-specific RNA-seq of poly(A)+ RNA from LNCaP prostate cancer cells showed transcription from the plus strand in the RASSF1 locus (Figure 1C), and the assembly of these RNA-seq reads using the Cufflinks tool generated a consensus sequence mapping to the genomic plus strand in the locus. The gene discussed is RASSF1; the disease is Familial prostate cancer.