The in vitro tests (ImmunoCap allergy assays) showed lower frequencies of allergy, with 18.3% of the participants having elevated IgE against inhalation-related allergen antigens using the Phadiatop assay, and 11.6% having elevated levels of IgE against food-related allergens using the F×5 assay (Fig. 1A and B, respectively). This evidence concerns the gene IGHE and allergic disease.