Tumor-associated CXCR3+ Treg were mostly Helios-positive, and T-bet+ Treg could be generated in vitro by culturing CD45RA+CCR7+ rTreg (mostly containing tTreg) under Th1-polarizing conditions (157), suggesting their derivation from committed tTreg. The gene discussed is CXCR3; the disease is neoplasm.