To determine if HIV-1 genes are differentially expressed at the protein level in Tg mice at different stages of lymphadenopathy, proteins from splenocytes isolated from 3 WT, asymptomatic Tg mice without skin lesions (Tg no S), Tg mice at early stages of lymphadenopathy (Tg pre-L), and Tg mice at late stages of lymphadenopathy (Tg L) were subjected to SDS-PAGE and immunoblotting with anti-HIV antibodies specific for p17 and nef (Figure 2C). Here, S100B is linked to Lymphadenopathy.