In β-gal staining assay, after 72 h of transfection, a lower proportion of senescent cells was observed in the group transfected with GNAO1 siRNA compared with that in the group of NC and NTC (Figure 4), indicating that down-regulation of GNAO1 inhibited the senescence of HCC cells. The gene discussed is GNAO1; the disease is hepatocellular carcinoma.