Sun and Rigas [56] went on to demonstrate that proapoptotic inhibition of NF-κB signalling in human colon cancer cell lines, treated with NO-aspirin, stemmed from the latter's induced generation of reactive oxygen and nitrogen species (RONS), which may have interacted directly or indirectly (via the redox-sensitive thioredoxin (TRX) system) with NF-κB, impairing its ability to bind to recognition DNA sequences in the nucleus. The gene discussed is NFKB1; the disease is colonic neoplasm.