Our previous in vitro data on WIN-34B has shown cartilage protective effects through the regulation of matrix proteinases (aggrecanases and MMPs/TIMPs), inflammatory mediators (PGE2, NO, IL-1β, and TNF-α), and the MAPK pathways in osteoarthritis human cartilage explants culture and chondrocytes [15]. This evidence concerns the gene TNF and osteoarthritis.