Through the retrospective study on the 108 patients with HCC, we found that the degree of differentiation in the AFP-negative group was higher than that in the AFP-low and the AFP-high groups, and the vascular infiltration rate in the AFP-negative group was lower than that in the other two groups, though the differences were not statistically significant between the AFP-low group and AFP-high group. The gene discussed is AFP; the disease is hepatocellular carcinoma.