To investigate the level of PCAF in HCC cell lines and select the appropriate cell models for the further experiment, we detected the mRNA and protein expression of PCAF in Hep3B, HepG2, Huh7, PLC/PRF/5 and SKHep1 cells by qRT-PCR and immunoblotting. The gene discussed is KAT2B; the disease is hepatocellular carcinoma.