In contrast with the DENV-3-infected WT mice, 100% of the Nos2−/− mice survived the infection, without exhibiting any clinical signs until the 30th d.p.i. To verify whether the virus replicates in the brains of Nos2−/− mice, these mice were infected with 4 × 102 pfu of DENV-3 genotype I and euthanized on the 8th d.p.i., which corresponds to the peak of viral replication in the WT mouse brains. Here, NOS2 is linked to infection.