We confirmed MFGE8 protein secretion by ELISA analysis of the conditioned medium of two of the mRNA-expressing cell lines: SKOV-3 and IGROV-1, and we identified another ovarian carcinoma cell line, SHIN-3 [14], which did not secrete detectable levels of MFGE8 in vitro (Figure 2b). Here, MFGE8 is linked to ovarian carcinoma.