ACTA1 and nemaline myopathy: Considering the rarity of NM, the well-known limitations linked to the analysis of biopsy samples and the possible differences between in vitro and in vivo measurements of muscle function, we aimed at investigating in vivo the functional, anatomical and metabolic consequences of the ACTA1 Asp286Gly mutation in a recently generated transgenic NM mouse model by utilising a strictly noninvasive approach.