To investigate roles of SIX3 in NSCLC cells where SIX3 was methylation-silenced, we established stable NSCLC cell lines after transfection with SIX3 cDNA and subsequent G418 selection, and confirmed restoration of SIX3 expression in these stable transfectants (Fig. 3a, SIX3 expression in normal adult lung tissues was used as a reference). The gene discussed is SIX3; the disease is non-small cell lung carcinoma.