On the other hand, although Alizarin red S staining for matrix mineralization (Fig. 3C, Fig. S1B) concomitant with osteoblast-specific genes alkaline phosphatase (ALP) and osteocalcin (OC) (Fig. 3D) expression showed a minor increase in the osteogenic activity of cancer-associated 3A6 derivatives, a reverse trend between osteogenic and adipogenic differentiation was found among these cell lines. This evidence concerns the gene BGLAP and cancer.