Our results, firstly demonstrate an association of rs4640525APPL1 G/C and rs11112412 -APPL2 GA variants with NAFLD being the A allele at the APPL2 locus and the C allele at the APPL1 locus more represented among NAFLD subjects in comparison to controls. The gene discussed is APPL1; the disease is metabolic dysfunction-associated steatotic liver disease.