Next, by performing Western blot analysis of the soluble protein- and membrane-protein-enriched cellular fractions (SF, MF) and of subcellular protein fractions (cytosolic, membrane, nuclear, and cytoskeletal), we examined whether the potentially PD-associated variant p.A350V of SLC41A1 was properly targeted into the cytoplasmic membrane, as demonstrated for wt SLC41A1 [20], [27]. Here, SLC41A1 is linked to Parkinson disease.