Some studies have supported the need for using highly sensitive molecular techniques in MM such as pyrosequencing and allele specific-q-PCR, that was supported by testing their sensitivity on serial dilutions of DNA extracted from a cell line carrying a heterozygous BRAF c.1799T>A (V600E) mutation [24], [25], [26], [39], [40]. The gene discussed is BRAF; the disease is Miyoshi myopathy.