During optimisation of a plasma membrane protein enrichment strategy based on cell surface biotinylation and avidin affinity chromatography for a comparative study (7) using the VHL-defective UMRC2- renal cancer cell line as a model system, purified proteins were catalogued using 1D PAGE followed by in-gel tryptic digestion and LC-MS/MS (GeLC-MS/MS). Here, VHL is linked to renal carcinoma.