BDNF and depressive symptom measurement: In this study, the serum BDNF levels are significantly lower in T2DM patients carrying either 66Met homozygote or 66Val/Met heterozygote compared with that carrying 66Val homozygote, indicating that this homozygosity for the 66Met allele confers an increased risk for depression in T2DM patients through mechanism involving reduced BDNF production and secretion.