Because c-Myc and NFkB are among the top reciprocal transcription factors in the migrating population, we assessed the clinical validation of the activation status of these transcription factors by measuring the levels of activated c-Myc (phospho c-Myc [37], [38]) and activated NF-κB (phospho p65/NF-κB [39]) on the invasion GBM TMA. The gene discussed is RELA; the disease is glioblastoma.