These data demonstrate the levels of activation of transcription factors c-Myc and NF-κB in the two subpopulations of GBM cells with higher NFkB activation detected in the cells at the invading rim, and elevated c-Myc activation in cells residing in the tumor core in situ, corroborating the outcome of in vitro transcription factor profiling study. The gene discussed is MYC; the disease is glioblastoma.