To ensure that abnormal glucose uptake is specific for TDP-43 over-expression and not related to expression of a toxic ALS related protein in general, we performed the 2-NBDG assay in FDB muscle fibers isolated from transgenic mice expressing G93A Cu, Zn superoxide dismutase (SOD1) which causes familial ALS type 1 [25]. This evidence concerns the gene SOD1 and amyotrophic lateral sclerosis.