The combination of RT-LAMP with the dengue IgM and IgG ELISA resulted in a significant increase (p < 0.001) in sensitivity to 97.7% (167 of 171) in comparison to using dengue IgM and IgG ELISA alone which had sensitivity of 70.8% (121 of 171) (Figure 5). This evidence concerns the gene CD40LG and dengue disease.