In vivo 4T1 cells metastasis in the lung was markedly reduced by OGT shRNA, and inhibition of E-cadherin expression in these cells (shOGT + shCadherin) restored invasiveness to control levels, suggesting an important role for regulation of E-cadherin expression by O-GlcNAc in cancer cell invasiveness (31). This evidence concerns the gene OGT and cancer.