The aortic valve surface from all strains, ApoE4-TR, ApoE3-TR and WT mice, on a HF diet appeared to have an intact covering with smooth endothelial cell layer as demonstrated by H & E staining (Figure 5A,B,C, respectively) and resembled the aortic valve surface of WT mice fed with normal chow (Figure 5D). This evidence concerns the gene APOE and hydrops fetalis.