We performed loss-of-function studies in the 4T1 mammary tumor model, which is driven by TGF-β.33 Knockdown of C/EBPβ in 4T1 cells through overexpression of Cebpb shRNA resulted in decreased expression of E-cadherin, and increased expression of vimentin (Figure 5a). This evidence concerns the gene TGFB1 and breast cancer.