Consistent with our model that LUBAC is not a negative regulator of MAVS signaling, tetracycline-inducible knockdown of HOIP in the human osteosarcoma cell line U2OS modestly inhibited the activation of IRF3 and induction of IFNβ by VSV (Figure 3—figure supplement 2A–C). The gene discussed is RNF31; the disease is osteosarcoma.