Because a large number of T. cruzi proteins involved in host-parasite interactions such as members of the large trans-sialidase, mucin and MASP families are GPI anchored, the availability of T. cruzi cell lines with disrupted genes of the GPI biosynthetic pathway would allow us to perform a number of studies regarding the effect of the absence of these proteins on the parasite surface during infection. This evidence concerns the gene MUC5AC and infection.