In our previous proof-of-concept study [6], we have reported that a 3 hour pre-treatment with choline+PNU-120596 significantly delayed anoxic depolarization/injury of hippocampal CA1 pyramidal neurons in the complete oxygen/glucose deprivation model of ischemic stroke in acute hippocampal slices and activation of α7 nAChRs was required; while intravenous administration of PNU-120596 30 min post-ischemia in the MCAO model of ischemic stroke significantly reduced cerebral infarct volume [6]. The gene discussed is CHRNA7; the disease is ischemic stroke.