As a result, 8 genes related to the Wnt/β-catenin signaling pathway (Lef1, Nkd1, Ccnd1, Sfrp2, Fosl1, Wnt5A, Wif1 and Fgf4) were identified as up-regulated in KO tumors compared to 24-month-old WT tumor-free livers, while another 3 genes from the pathway (Ctnnbip1, Wnt2 and Sfrp1) were down-regulated in KO tumors (Fig. 2). Here, CCND1 is linked to neoplasm.