In conclusion, we show here for the first time that (1) PPARα is upregulated in high grade RCC tissues compared to low grade tissues, (2) PPARα inhibition attenuates RCC cell viability through c-Myc, CDK4, and cyclin D1 decrease mediated cell cycle arrest and apoptosis induction, and (3) glycolysis inhibition synergizes with PPARα against cell viability. The gene discussed is CDK4; the disease is renal cell carcinoma.