In conclusion, we show here for the first time that (1) PPARα is upregulated in high grade RCC tissues compared to low grade tissues, (2) PPARα inhibition attenuates RCC cell viability through c-Myc, CDK4, and cyclin D1 decrease mediated cell cycle arrest and apoptosis induction, and (3) glycolysis inhibition synergizes with PPARα against cell viability. This evidence concerns the gene MYC and renal cell carcinoma.