To further verify if miR-185 and 342 directly bind with 3′ UTRs of SREBP-1 and SREBP-2, we performed 3′ UTR luciferase reporter assay and found that the relative 3′ UTR luciferase activities of both SREBP-1 and SREBP-2 were significantly decreased in miR-185 and 342 transfected prostate cancer cells (Fig. S1B). This evidence concerns the gene SREBF1 and Familial prostate cancer.