INSR and breast carcinoma: To assess expression of IR and IGF-2 in human endometrial cancer cell lines, reverse transcription polymerase chain reaction (RT-PCR) and Real time RT-PCR were performed using RNA from the HEC-1-A, Ishikawa, KLE, RL95-2-CON endometrial cancer cell lines, positive control Liver cancer cell line Hep-G2 and breast cancer cell line MCF-7.