Previous studies have reported that IR-A is a high affinity receptor for IGF-2 [10], To further confirm whether the endometrial cancer cell lines can secrete IGF-2, enzyme-linked immunosorbent assay (ELISA) was performed using cell culture supernatants from the HEC-1-A, Ishikawa, KLE, RL95-2-con and RL95-2-IR-A cell lines. This evidence concerns the gene IGF2 and endometrial cancer.