In contrast, tumor cells pre-treated with AG1024, Erlotinib, or both showed a delayed H2AX phosphorylation peak at 4 hour, and continuous H2AX phosphorylation till 24 h after irradiation, suggesting impairment of DSB repair after AG1024 and Erlotinib treatment (Figure 2A, B). This evidence concerns the gene H2AX and neoplasm.