We therefore chose to use the B. pertussis calmodulin-dependent adenylate cyclase (CyaA) reporter system [28], which has previously identified substrates of the VirB T4SS of Brucella[20], at an infection time point (6 h pi) where MOI adjustments yielded similar numbers of recoverable CFUs for both wild type and VirB-deficient bacteria (Fig. S3B). The gene discussed is ADCY1; the disease is infection.