In the present study, the VEGF expression level in the patients with lymph node metastasis was 85.7%, significantly higher than that in the patients without lymph node metastasis (40%; P= 0.0009) However, there was no significant difference between the patients with hepatic metastasis, which was possibly associated with the smaller number of cases of patients with hepatic metastasis. This evidence concerns the gene VEGFA and metastatic malignant neoplasm in the lymph nodes.