ERBB2 and metastatic malignant neoplasm in the lymph nodes: HER2 gene amplification in ESCC was markedly correlated with the tumor local infiltration, venous invasion and lymph node metastasis (χ2=4.789, 3.858 and 5.354, respectively; all P<0.05), but was not correlated with the gender, age and tumor differentiation of the patient.