To determine whether ERManI is a bona fide target of miR-125, we transfected control, miR-125a, or miR-125b mimics at different concentrations into PLC/PRF5 cells, a hepatoma cell line that expresses high levels of ERManI, and examined the changes in ERManI expression by western blotting. This evidence concerns the gene MAN1B1 and hepatocellular carcinoma.