Although our tissue staining results were further supported by the upregulation of ERManI in hepatoma cell lines, it would be ideal to determine the level of upregulation in HCC tissues using a more quantitative method such as quantitative western blotting since immunohistochemistry is semi-quantitative, and can be affected by the antibody reactivity upon retrieved antigens. The gene discussed is MAN1B1; the disease is hepatocellular carcinoma.