When the cutoff value was set as the group III (with low α-SMA and high MVD) versus other threes groups, the negative predictive value, positive predictive value, sensitivity, and specificity of HCC cohort 1 were 0.749, 0.616, 0.491, 0.832 for OS and 0.616, 0.558, 0.364, 0.780 for RFS, and of PC cohort 2 were 0.690, 0.600, 0.409, 0.829 for OS and 0.476, 0.733, 0.333, 0.833 for RFS, respectively. Here, ACTA1 is linked to pachyonychia congenita.