Not surprisingly, miR-21 gene expression was markedly reduced (∼70%), while the expression of PTEN and PDCD4 (miR-21-target genes) was enhanced in tumor tissue from EF24-treated mice, which is similar to the effect of EF24 on miR-21 gene expression in DU145 cells in vitro (Figure 6C). This evidence concerns the gene PDCD4 and neoplasm.