AKT1 and acute myeloid leukemia: Consistent with the findings of the antibody array, the level of phosphorylated Akt (Serine 473) in western blotting assay was also significantly higher in all AML-M5 derived cell lines (Fig. 2B) as well as in multiple AML-M5 patient samples in which N-CoR was lost (Fig. 2C).