Furthermore, TRAV10 gene encoding Vα24 domain of iNKT cells and its mRNA expression level has significant correlation with the number of iNKT cells [14], [22], so we measured the expression of TRAV10 gene mRNA by qRT-PCR to evaluate the prognostic value of iNKT cells for HCC patients on the basis of long-term extremely low temperature preserved specimens. The gene discussed is TRAV10; the disease is hepatocellular carcinoma.