Since attenuation of pro-inflammatory cytokines secretions is IBD therapeutic targets, In this study, we co-cultured human epithelial colorectal adenocarcinoma (Caco-2) cells with probiotics and then administered LPS, which induced TNF-α, IL-6, IL-8 and IL-12 secretion, to biologically mimic the inflammatory situation of IBD. The gene discussed is CXCL8; the disease is inflammatory bowel disease.