The obtained RQ values, calculated based on the delta-delta CT method, adjusted to the β-actin expression, and relative to the expression level of calibrator (for which RQ = 1), were compared among NSCLC patients in regard to histopathological NSCLC subtypes, tumor staging (TNM, AJCC), patients' age, gender, and smoking history, as well as to CTLA-4 polymorphic variants. This evidence concerns the gene ACTB and neoplasm.