The concentrations of MCP-1 (P<0.05), GM-CSF (P<0.01), G-CSF (P<0.01), MIP-1α (P<0.01) and TGF-β1 (P<0.01) was significantly decreased in the spermatozoa-free seminal fluid recovered from infertile men group when compared to the normal fertile donors group. Here, TGFB1 is linked to Infertility.