INCA1 and infection: To test this hypothesis, we transgenically expressed wildtype IncA, Δ34-IncA, IncA1–141, Phe/Asp-IncA1–141, and Phe/Asp-IncA fused to the monomeric form of DsRed in HeLa cells 24 h prior to infection with C. trachomatis strain L2 and monitored changes in inclusion morphology associated with each mutant.