The large difference between the levels of neutralizing antibodies generated by vaccination of guinea pigs with Al(OH)3 and either rLTB/HCC/H CD or rH CC/H CD support these findings and indicate that LTB indeed works as a systemic humoral immune response adjuvant, even though it was originally described to be a mucosal adjuvant [47]. This evidence concerns the gene LTB and hepatocellular carcinoma.