MECP2 and Rett syndrome: We tagged various MeCP2 subdomains and regions spanning the MBD, TRD, the C-terminus and MeCP2 lacking its C-terminus (ΔC-terminus) or mutant MeCP2R133C (Rett Syndrome point mutation in the MBD domain) [54] to EGFP-lacR to identify which MeCP2 subdomain is responsible for unfolding of the lacO chromosomal domain upon targeting (Figure 3A).